Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
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چکیده
منابع مشابه
Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
Animal cells and cell lines, such as HEK-293 cells, are commonly cultured at 37°C. These cells are often used to express recombinant proteins. Having a higher expression level or a higher protein yield is generally desirable. As we demonstrate in this study, dropping culture temperature to 33°C, but not lower, 24 hours after transient transfection in HEK-293S cells will give rise to ~1.5-fold h...
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................................................. 3 Background.............................................. 4 Materials and Methods...................................8 Results....................................................12 Discussion................................................14 Acknowledgments.......................................16 References.........................................
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The human embryonic kidney 293 (HEK-293) cells are commonly used as host for the heterologous expression of membrane proteins not least because they have a high transfection efficiency and faithfully translate and process proteins. In addition, their cell size, morphology and division rate, and low expression of native channels are traits that are particularly attractive for current-voltage mea...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2015
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0123562